PseudomonasÂ and risk factor mitigation for chronic lung allograft dysfunction
Allan R.Â Glanville
European Respiratory JournalÂ 2020Â 56:Â 2001968;Â DOI:Â 10.1183/13993003.01968-2020
Univariable analysis supports attempts at “eradicating”Â PseudomonasÂ from bronchopulmonary secretions after lung transplantationÂ https://bit.ly/2Nw6h5P
Unlike binary logic, natural circumstances are more complex and demand a pluralistic view of causality, notwithstanding the recognition that in systems of coexistence, mitigation of deleterious outcomes can still be achieved by reducing one of the driving factors in the equation. So it is with airway disease, both large and small, after lung transplantation. Mucosal and structural damage promote an unhealthy local milieu with impaired barrier function, compromised local immunity and reduced efficacy of mucociliary transport. Perturbations of the pulmonary microbiome along with chemical damage from often occult gastric aspiration set the foundation for such a scenario. However, unlike fibrotic luminal occlusion of small airways typical of obliterative bronchiolitis, which is permanent, the damage described above may be amenable to change. Of course, in a parallel causation model, the damage described may well alter the local airway environment to facilitate the processes of airway restructuring and, ultimately, loss. So, with this conceptual overview, the attempt by the team from Leuven to “eradicate” airwayÂ PseudomonasÂ can be seen as a potentially worthwhile endeavour, but does it work and are the claims robust? Let us examine the factors critical to the claims. Firstly, the claim of eradication ofÂ PseudomonasÂ is based on examination of airway secretions, sputa and, where available, from scheduled and clinically mandated bronchoalveolar lavage (BAL) fluid culture. Clearly, the sensitivity of these different tools varies greatly and the true negative predictive value is unknown, but clinical experience cautions enthusiasm regarding the ability of eradicating a lower airway pathogen, particularly when biofilms and structurally abnormal airways coincide to protect microdeposits of infection. That is not to say that reducing the colonising load, or perhaps more accurately, the infecting load ofÂ PseudomonasÂ is not a worthwhile goal as outlined above. Indeed, whetherÂ PseudomonasÂ is ever a benign colonising organism in the transplanted lung is open to serious debate, which promotes a genuine attitude that an attempt at eradication is worthwhile provided the therapy is not worse than the disease! A long-term risk/benefit view is mandated especially as anti-PseudomonasÂ therapies risk development of more resistant organisms leading to attempts at less effective and more expensive therapies. Similarly, and given that mostÂ PseudomonasÂ infection is hospital acquired, a case may be made for reducing hospital exposure and invasive surveillance procedures unless mandated for specific diagnostic purposes. Secondly, the time interval to determine success of eradication was necessarily arbitrary (6â€…months) and the therapies employed were not standardised over time, reflecting one of the common limitations of single-centre retrospective studies. Of interest, 23/76 subjects in the successful eradication group did not appear to undergo specific therapies and, conversely, none of the unsuccessful group had a course of quinolone therapy, as described in theÂ supplementary dataÂ (table S2). As interesting as this may be from a pathophysiological perspective, it does not change the ultimate conclusion that patients in whom the “presence” ofÂ PseudomonasÂ could not be detected on respiratory samples had a longer CLAD-free survival and graft survival. Noting this point, it is worth remembering that many registry reports undercount the frequency of CLAD and overcount the CLAD-free period post-transplant, as recently reported by KulkarniÂ et al.. Most lung transplant recipients die or develop bronchiolitis obliterans syndrome (BOS), as the obstructive phenotype CLAD was termed prior to the current appellation, within 4â€…years. Very few remain alive and free from BOS at 10â€…years .
Thirdly, given the multiple risk factors for CLAD, including acute cellular rejection and antibody mediated rejection, do we accept the proposition thatÂ PseudomonasÂ is indeed an independent risk factor for CLAD rather than just a marker of damaged airways? Once again, the evidence is largely based on single-centre retrospective studies with some illuminating scientific insights into mechanisms. BothaÂ et al.Â in 2008 concluded from a study of 155 consecutive lung transplant subjects thatÂ de novoÂ colonisation of the lung allograft byÂ PseudomonasÂ was strongly associated with the subsequent development of BOS within 2â€…years of transplant (23.4%Â versusÂ 7.7% colonisedÂ versusÂ not colonised, p=0.006). Work from ValentineÂ et al.Â in 2009 was in accord but emphasised the importance of pneumonia as a harbinger of BOS. MainzÂ et al., in 2012, demonstrated identicalÂ P. aeruginosaÂ genotypes in the upper airways of patients with cystic fibrosis (CF) prior to and after lung transplant, underlining the risk of upper respiratory tract as a source for colonisation of the transplanted lungs. In support of these findings, BeaumeÂ et al.Â in 2017 showed thatÂ PseudomonasÂ invaded the host microbiota within 3 days of transplant, with a reduction in richness and diversity of species. Despite antibiotic therapy,Â PseudomonasÂ dominated the allograft microbiota until postoperative day 95, perhaps augmented by phenotypic adaptation resulting in increased biofilm formation and swim motility capacity.
BorthwickÂ et al.Â , in a 2011 study of primary bronchial epithelial cells isolated from lung transplant recipients, provided evidence thatÂ P. aeruginosa,Â viaÂ the activation of monocytic cells, could accentuate transforming growth factor-Î²1-driven epithelial-to-mesenchymal transition which has been implicated in dysregulated epithelial wound repair that contributes to obliterative bronchiolitis after lung transplantation. Using a Cox semi-Markovian approach, GregsonÂ et al. in 2013, showed that the likelihood of transition from transplant to BOS was increased by acute rejection, chemokine ligand (CXCL)5, and the interaction betweenÂ PseudomonasÂ and CXCL1. In this study, theÂ PseudomonasÂ effect was due to infection rather than colonisation. Evidence challenging the benefit of clearance ofÂ PseudomonasÂ has been demonstrated in the CF population by BurkettÂ et al.Â in 2012, who showed that declines in lung function, rates of pulmonary exacerbations, deaths or lung transplant were not different between patients who clearedÂ PseudomonasÂ compared to those who remained persistently infected. Indeed, no clinical benefit was identified in patients who clearedÂ PseudomonasÂ from sputum compared to those who remained persistently infected. The critical difference likely resides in the innate immune response to the allograft (acute and chronic rejection)Â versusÂ the response of native airways to infection.
New techniques are now available to map all components of the human pulmonary microbiome and are beginning to inform our understanding. In studies of the microbiome after lung transplant, DicksonÂ et al.Â in 2014 discovered that a distinct species,Â P. fluorescens, was often identified in asymptomatic transplant subjects by pyrosequencing but not detectedÂ viaÂ the standard bacterial cultures used in the current study, having unique genomic and microbiologic features and widely divergent clinical associations fromÂ P. aeruginosa. Following on from these studies, BernasconiÂ et al. in 2016 showed that “inflammatory” and “remodelling” profiles were linked to bacterial dysbiosis through mechanistic assays in macrophages, whereas a balanced microbial community maintained homeostasis. In the same year, BorthwickÂ et al. measured alarmins in serial BAL samples and showed that interleukin-1Î± was increased in BAL samples of lung transplant recipients growingÂ P. aeruginosaÂ and was significantly elevated within 3â€…months of developing BOS. In short, the case for the risk ofÂ P. aeruginosaÂ after lung transplantation is well made, but evidence for the benefits of attempts at “eradication”, or indeed a reduction in microbial load, is not overwhelming. Hence the current study provides most useful information to guide practice. A number of questions remain, including the utility of aerosolised therapies, which were not employed in this study as they are not reimbursed in Belgium after lung transplantation, despite their use in CF patients to reduce microbial load and hence frequency and severity of acute exacerbations. Also, whether concurrent antibiotic therapy at the time of sample acquisition may have impacted results is not explicitly stated but given the small number of patients in whomÂ PseudomonasÂ was not “eradicated” likely would have an insignificant biasing effect. The authors have attempted to resolve the matter of retrospective assignation of groups for Kaplanâ€“Meier analysis by using a segmented time-based approach (figure 4), where the benefits of aÂ Pseudomonas-free respiratory tract appear obvious but the numbers are small (4â€“8 per annum) which serves to magnify the effect. The proper handling of multiple time-dependent effects after lung transplant remains somewhat problematic.
In summary, the goal of eradication ofÂ PseudomonasÂ after lung transplant, as a strategy to reduce the devastating impact of CLAD, now has a building evidence base but as ever, larger, prospective, multicentre studies examining multivariate risks and benefits as well as costâ€“utility and other strategies to mitigate CLAD are needed, without which the sterling work of these investigators may not be fully appreciated as anything more than the brave beating of a distant drum.
Conflict of interest: A.R. Glanville has nothing to disclose.
- ReceivedÂ May 24, 2020.
- AcceptedÂ June 22, 2020.
- Copyright Â©ERS 2020
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